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BotanyNEET 2024DNA structure and replication

Molecular Basis of Inheritance

Which of the following statements is correct regarding the process of replication in E. coli?

  1. A.The DNA-dependent RNA polymerase catalyses polymerisation in one direction, that is 5′ → 3′
  2. B.The DNA-dependent DNA polymerase catalyses polymerisation in the 5′ → 3′ as well as the 3′ → 5′ direction
  3. C.The DNA-dependent DNA polymerase catalyses polymerisation in the 5′ → 3′ direction
  4. D.The DNA-dependent DNA polymerase catalyses polymerisation in one direction, that is 3′ → 5′

Solution

DNA-dependent DNA polymerase adds nucleotides only in the 5′→3′ direction — on the leading strand continuously and on the lagging strand as Okazaki fragments. The 3′→5′ direction is never synthesised.

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