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ZoologyNEET 2025Tools of recombinant DNA technology

Biotechnology: Principles and Processes

Which of the following enzymes are not essential for gene cloning?

A. Restriction enzymes · B. DNA ligase · C. DNA mutase · D. DNA recombinase · E. DNA polymerase

Choose the correct answer from the options given below:

  1. A.C and D only
  2. B.A and B only
  3. C.D and E only
  4. D.B and C only

Solution

Gene cloning needs restriction enzymes to cut, DNA ligase to join and DNA polymerase for PCR. 'DNA mutase' and 'DNA recombinase' are not part of the toolkit. Hence C and D.

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