Chapter 233 of 2363 min read
Processes: cloning, PCR, bioreactors
Why this is asked: PCR only survives cycle after cycle because Taq polymerase, isolated from Thermus aquaticus (a bacterium living in hot springs), survives the roughly 95°C denaturation step intact — an ordinary DNA polymerase would be destroyed at that temperature and need replacing every cycle, which is why the source organism itself, not just "a polymerase is used," is the specific fact NEET asks for.
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