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BotanyNEET 2021Tools of recombinant DNA technology

Biotechnology: Principles and Processes

Plasmid pBR322 has a PstI restriction enzyme site within the gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for galactoside production and the recombinant plasmid is inserted in an E. coli strain:

  1. A.It will not be able to confer ampicillin resistance to the host cell✓
  2. B.The transformed cells will have the ability to resist ampicillin as well as produce galactoside
  3. C.It will lead to lysis of the host cell
  4. D.It will be able to produce a novel protein with dual ability

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